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Topic: Segmentation of images...contour detection problem
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Md. Shahriar Karim

Posts: 54
Registered: 2/2/09
Segmentation of images...contour detection problem
Posted: Oct 25, 2010 1:49 PM
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Hi All,

I am actually trying to use Image Segmentation method to find cell boundaries from some images that contain the time evolution of the cell division process. Can any one suggest some ideas to proceed forward?

Part of my process is as below:

B(:,:,i) = double(imread('trial_stack.tiff',i));
B_dp(:,:,i) = medfilt2(mat2gray(B(:,:,i)));
BW(:,:,i) = im2bw(B_dp(:,:,i),0.4);
B_product = B_product.*(4*BW(:,:,i)+1);
imshow(mat2gray(BW(:,:,i)))

Finally, I use "edge" matlab function, however, didn't get any good result at the end. "Edge" part is as follows--

------------------------------------------------------------------------------------------
Mean_I = imshow(mat2gray(sum(double(B_dp),3)));
figure(55)
I = mat2gray(B_product.^(1/100));
imshow(I)

figure(56)
background = imopen(I,strel('disk',7));

I2 = I-background;
imshow(I2)
figure(57)
I2 = medfilt2(I2);
pave_BW = edge(I2,'canny',0.4);
imshow(pave_BW)
-------------------------------------------------------------------------------------------

I also have uploaded the stack images (49 part, look read when you open the link) and also a final version after I use the edge command. Please let me know if you have any way forward to have better contour detection so that a clear movie out of all these 49 images can be made.

http://drop.io/i6fmldv

Can any one suggest something on Image Segmentation that could probably help to get a perfect contour detection from all these images?

Thanks in advance.

Shahriar




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