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Topic: Analyse CLSM Image to get Particle density
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Ramona

Posts: 1
Registered: 9/3/13
Analyse CLSM Image to get Particle density
Posted: Sep 3, 2013 2:30 PM
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Hallo!

For a project I have to analyze pictures form CLSM (Confocal Laser Scanning Microscope). In the pictures are green Particles and I have to find out their spatial distribution (x,y,z and Intensity).

Therefore I worked with imageJ to measure the particles in a grid of 300 µm mesh size. Then I calculated the Area fraction of the particle (intensity) and get a column with the Data related to x and y coordinates and the stack (z) where I measured in.

Now I want to plot the Data in Matlab in a 3d-plot (with contourslice like in dokumentation http://www.mathworks.de/de/help/matlab/visualize/techniques-for-visualizing-scalar-volume-data.html?searchHighlight=scalar+volume+data), but I see, that I need a different way to explain my data (like a matrix not columns).

My question: Is it possible to make such an image-analysis in matlab too? So I can load the picture and get some kind of particle density or area fraction? I also need the matrix or array for more calculations.

Thank you!

Cheers

Ramona



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