For a project I have to analyze pictures form CLSM (Confocal Laser Scanning Microscope). In the pictures are green Particles and I have to find out their spatial distribution (x,y,z and Intensity).
Therefore I worked with imageJ to measure the particles in a grid of 300 µm mesh size. Then I calculated the Area fraction of the particle (intensity) and get a column with the Data related to x and y coordinates and the stack (z) where I measured in.
My question: Is it possible to make such an image-analysis in matlab too? So I can load the picture and get some kind of particle density or area fraction? I also need the matrix or array for more calculations.