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Ramona
Posts:
1
Registered:
9/3/13


Analyse CLSM Image to get Particle density
Posted:
Sep 3, 2013 2:30 PM


Hallo!
For a project I have to analyze pictures form CLSM (Confocal Laser Scanning Microscope). In the pictures are green Particles and I have to find out their spatial distribution (x,y,z and Intensity).
Therefore I worked with imageJ to measure the particles in a grid of 300 µm mesh size. Then I calculated the Area fraction of the particle (intensity) and get a column with the Data related to x and y coordinates and the stack (z) where I measured in.
Now I want to plot the Data in Matlab in a 3dplot (with contourslice like in dokumentation http://www.mathworks.de/de/help/matlab/visualize/techniquesforvisualizingscalarvolumedata.html?searchHighlight=scalar+volume+data), but I see, that I need a different way to explain my data (like a matrix not columns).
My question: Is it possible to make such an imageanalysis in matlab too? So I can load the picture and get some kind of particle density or area fraction? I also need the matrix or array for more calculations.
Thank you!
Cheers
Ramona



